Description
The IBE cryobank preserves plant organs in liquid nitrogen, at -196 °C, specifically: (i) polyembryonic seeds from 24 accessions of Citrus spp. from the Medicean Collection of Villa Reale di Castello, Florence, dating back to the XVI century; (i) dormant buds from 19 accessions of ancient varieties from the Veneto region, Italy; and (iii) dormant buds from 3 accessions of plum (Prunus domestica) from the C.A.V.-Center of Nursery Activities of Tebano, Ravenna. The cryo-procedures utilized for cryopreservation are the desiccation and direct immersion in liquid nitrogen of polyembryonic seeds (Citrus) and the dormant bud technique for the conservation of uni-nodal segments of Malus domestica and Prunus domestica. The ultimate goal is the long-term maintenance of ancient fruit tree germplasm. Tissue culture and micropropagation are the techniques used for germplasm recovering and propagation.
Taxonomic Coverage
Malus domestica, Prunus, spp. Citrus aurantium, Citrus sinensis, Citrus limon, Citrus limonimedica, Citrus medica, Citrus ×meyeri, Citrus ×myrtifolia, Citrus deliciosa, Citrus limetta, Citrus limonia, Citrus lumia, Citrus maxima, Citrus sp.
