DiSSCo-ITINERIS Metadata Catalog

Microbiology; genomics; metabolomics. Microbiology: growth and manipulation of bacteria. Molecular Biology: isolation and manipulation of nucleic acids; cloning and heterologous expression of genes of interest in model organisms (E. coli); gene expression analysis (RT-PCR, RNA-seq). Biochemistry: structural and functional analysis of proteins by SDS-PAGE, native-PAGE and western blot; UV-visible, fluorescent and circular dichroism spectroscopy; enzymatic assays (varius enzymes inclucing DNA polymerases); in vitro DNA-ligand interaction techniques by innovative techniques, as the Surface Plasmon Resonance (SPR) and Bio-Layer Interferometry (BLI); fluorescence microscopy for in vivo study of bacteria, nematodes, and plants.

Name: Rhizobacteria promoting plant growth and bacteria degrading toxic pollutants (CNR-IBBR-SUB)
Identifier: bf6ef975-8cde-47ea-9037-9ca01d8d61ea
Specimen Preservation Method: GLYCERIN

ITINERIS - Italian Integrated Environmental Research Infrastructures System | BIOMEMORY

Identifier: PNRR Project IR0000032 | CNR SAC.AD002.173

Content: ITINERIS (PNRR Project IR0000032) Activity 6.5 "Mining and mapping the functional biodiversity in vivo and ex-situ research collections" - This activity is aimed to provide biodiversity (meta)data linked to Natural Science Collections (NSC). Extended digital specimens will be linked to related information (e.g.. images and videos, codification of functional traits, DNA and protein sequences, environmental parameters, etc.) in the frame of a national network of biological research collections, including all the existing in vivo and ex-situ collections of biological organisms (animals, plants, fungi, algae, archaea, bacteria, viruses, etc.) which have been created and maintained through past and current research projects. Reference to genetic/genomic data for each specimen will allow for the identification of the most interesting material for further genetic/genomic analysis. Bio Memory (CNR-DiSBA Project No. SAC.AD002.173) is the network of biological collections of the Department of Biology, Agriculture and Food Sciences (DiSBA) of the National Research Council of Italy (CNR) for bio-monitoring, biodiversity conservation, agri-food and environmental sustainability, and human well-being. The project is aimed to create a network of biobanks (i.e., scientific research collections) where data and metadata associated to biological samples of different nature are collected and stored in a systematic and well-organized way. Maintaining the existing collections will allow their future use for a number of purposes, from the genetic improvement of organisms to face environmental changes (climate-ready organisms) to the fight against epidemics and pandemics affecting humans, animals and plants.

Funding: ITINERIS project no. IR0000032 — ITINERIS, Italian Integrated Environmental Research Infrastructures System—CUP B53C22002150006 (D.D. n. 130/2022) Funded by the EU—Next Generation EU—Mission 4 “Education and Research”—Component 2: “From research to business”—Investment 3.1: “Fund for the realisation of an integrated system of research and innovation infrastructures” || Bio Memory (CNR-DiSBA Project No. SAC.AD002.173) - CNR-DiSBA - Department of Bio-Agrofood Sciences, National Research Council of Italy