Overall records: 417
doi: https://doi.org/10.15468/k2pzpk
Record Count: 417
Dataset Type: Occurrence
Dataset subtype: Specimen
Description: The frozen collection consists of over 3,000 transgenic and mutant strains of non-parasitic nematodes, Caenorhabditis elegans species, accumulated over forty years. The collection, established in the early ’80s by Dr. Paolo Bazzicalupo, is kept in triplicate, is unique in Italy, and supports numerous Italian groups. C. elegans is the only multicellular animal model that can be frozen and stabs are stable for decades. Preservation techniques: Cryopreservation of L1 larval stage worms in 1 mL freezing solution containing Na Cl 0.1M, phosphate buffer pH 6.2, Glycerol 30%. Services: Distribution of strains to the Italian C. elegans community. Local conservation of strains from CAENORHABDITIS GENETICS CENTER (CGC). Genotyping and phenotyping. Competence / Expertise: Maintenance of C. elegans strains, Genetic crosses, Molecular biology, Anatomical methods, Neurobiology, Toxicology, Implementation of nutraceuticals, Genetic screens, Drug screens, Transgenesis. Quality: Genotyping and phenotyping of strains before and after freezing, including viability test. The collection is maintained as triplicate copies in 2 different -80° Ultrafreezers and 1 in L.N.
Keywords: Occurrence, Genetics, Animal model, Invertebrate, Non parasitic nematode, Specimen
Parent Collection: CNR-IBBR-CELITABASE
Parent Institution: CNR-IBBR-NA
Publisher: Consiglio Nazionale delle Ricerche, Istituto di Bioscienze e Bio Risorse (CNR-IBBR)
License: http://creativecommons.org/licenses/by-nc/4.0/legalcode